Estimation of Urinary 5-hydroxyindoleacetic Acid by Paper Chromatography--a Sensitive Method.

Creatine Kinase:AspartateAminotransferaseActivity Ratio as an Indicatorof the Source of an IncreasedCreatine Kinase Activity

7. Knight JA, Haymond RE. Improved colorimetry of urinary 3methoxy-4-hydroxyphenylaceticacid (homovanillic acid). Cliii Chem 1977;23:2007-10. 8. Ruthven CRJ, Sandler M. An improved method for the estimation of homovanullicacid in human urine. Clin Chiin Acts 1966;14:511-8. 9. Udenfriend S, Titus E, Weisabach H. The identification of 5hydroxy-indoleacetic acid in normal urine and a method for it...

A Method for Quantitative Extraction of Urinary 5-hydroxyindoleacetic Acid.

HE QUANTITATIVE ESTIMATION of urinary 5-hydroxyindoleacetic acid (5-HIAA) has attained clinical importance with increasing interest in indole metabolism. Urinary 5-HIAA levels have largely been determined by the use of an extraction procedure described by Udenfriend et al. (1). The author has found, as have others (2), that this method produces inconsistent results on varying dilutions of a giv...

The Application of Weak-Anion Exchange Chromatography for the Analysis of Organic Zwitterions Using LC/MS/MS

A rapid and accurate quantitative method was developed and validated for the analysis of four urinary organic acids with nitrogen containing functional groups, formiminoglutamic acid (FIGLU), pyroglutamic acid (PYRGLU), 5-hydroxyindoleacetic acid (5-HIAA), and 2-methylhippuric acid (2-METHIP) by liquid chromatography tandem mass spectrometry (LC/MS/MS). The chromatography was developed using a ...

Urinary 5-hydroxyindoleacetic acid by HPLC with electrochemical detection.

Automated determination of 5-hydroxyindolylacetic acid in urine by high-performance liquid chromatography

5-hydroxyindolylacetic acid (5-HIAA), a major serotonin cata-bolite, is eliminated in urine. In carcinoid tumours, 95 ofwhich occur in the small intestine, large quantities of serotonin are secreted by the entero-chromaffin cells of Kultchizky-Masson. Early detection of these tumours is essential, since unless they are surgically treated in the initial stages then their prognosis is serious. Ea...